| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Cross-amplification of heterologous microsatellite markers in Piracanjuba
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| Autores: |
Castro,Pedro Luiz de
Ribeiro,Ricardo Pereira
Santos,Silvio Carlos Alves dos
Goes,Elenice Souza dos Reis
Souza,Felipe Pinheiro de
Poveda-Parra,Angela Rocio
Vargas,Lauro
Urrea-Rojas,Angela Maria
Lopera-Barrero,Nelson Mauricio
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| Data: |
2017-12-01
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| Ano: |
2017
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| Palavras-chave: |
Brycon orbignyanus
Conservation
Molecular markers
Heterologous primers
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| Resumo: |
ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/0103-8478cr20170374
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.47 n.12 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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